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Chanchal DasGupta

PhD:
SINP (1974)
Area of Interest:
Biology
Email ID:
chanchaldg
Research Interest:

Research in Ph.D. and post doctoral level was on the involved of E.coli RecA protein  in SOS and recombination  function. Showed the involvement of RecA  in Weigle reactivation of single stranded DNA phage ØX-174 irrespective of damage chemistry of DNA in 1971, before SOS repair was postulated. Proposed and demonstrated the DNA single-strand transfer followed by paranemic heteroduplex  and Holliday crossover formation by the RecA protein in the laboratory of Prof. Charles Radding.
Since 1990, working on ribosome mediated protein folding and nascent protein mediated ribosomal subunit dissociation during translation. Both of these phenomena were proposed and demonstrated in this laboratory. The protein folding activity has been shown to be located at the peptidyl transferase center of the largest  ribosomal RNA (23S rRNA in E.coli) in bacterial, plant and animal cells and mitochondria. The peptidyl transferase center works as a universal protein folding mould. The nascent (or unfolded) protein mediated ribosome dissociation (in cis) to subunits is faster and more complete than the ribosome recycling factors mediated subunit dissociation shown from various other laboratories.

Academic Qualification:
  1. M.Sc. Physics, Calcutta University
  2. Ph.D. :  Biophysics Division, Saha Institute of Nuclear Physics, Kolkata, 1974
Positions:
  1. Post Doctoral Research : Yale University School of Medicine, New Haven, Connecticut, USA
  2. Professor, Department of Biophysics and Molecular Biology in Calcutta University during 1989-2009.
  3. Professor and Chairman, Department of Biological sciences, IISER-Kolkata since June 2009.
Awards and Honors:
  1. Elected Fellow, Indian Academy of Sciences, Bangalore.
  2. Elected Fellow, Indian National Science Academy, New Delhi.
  3. Elected Fellow, The National Academy of Sciences, Allahabad.
List of Publications:

Publications in International Journals

  1. R.K.Poddar and C.K.Das Gupta (1973) Ultraviolet reactivation of single stranded DNA phage  φX 174, Genetics, 74, Jun Supp., 214
  2. C.K.Das Gupta and R.K.Poddar (1975) Ultraviolet reactivation of single stranded DNA phage φX 174, Mol.Gen.Genet., 139, 77-91
  3. C.K.Das Gupta, A Vrancic and A.Guha (1977) Isolation of biotin operon of Escherichia coli, Gene, 1, 331-339
  4. C.K.Das Gupta and A.Guha (1978) Isolation of the regulatory segment of the biotin operon, Gene, 3, 233-246
  5. C.K.Das Gupta, RP.Cunninghum, T.Shibata and C.M.Radding (1979) Enzymatic cleavage of D.loop, Cold Spring Hatb Symp.Quant. BioI., 43, Part 2, 987-990
  6. T.Shibata, C.K.Das Gupta, R.P.Cunninghum and C.M.Radding (1979) Purified Escherichia coli RecA protein catalyzes homologous pairing of superhelical DNA and single stranded fragments, Proc.Natl.Acad.Sci.USA, 76, 1638-1642
  7. R.P.Cunninghum, T.Shibata, C.K.Das Gupta and C.M.Radding (1979) Single strands induce RecA protein to unwind duplex DNA for homologous pairing, Nature, 281,191-195
  8. T.Shibata, R.P.Cunninghum, C.K.Das Gupta and C.M.Radding (1979) Homologous pairing in genetic recombination: complexes ofRecA protein and DNA, Proc.Natl.Acad.Sci. , USA ,76,5100-5104
  9. R.P.Cunninghum, C.K.Das Gupta, T.Shibata and C.M.Radding (1980) Homologous pairing in genetic recombination: RecA protein makes joint molecules of gapped circular DNA and closed circular DNA, Cell, 20, 223-235
  10. T.Shibata, C.K.Das Gupta, R.P.Cunninghum and C.M.Radding (1980) Homologous pairing in genetic recombination: formation of D loops by combining action of RecA protein and a helix destabilising protein, Proc.Natl.Acad.Sci. , USA , 77,2606-2610
  11. C.K.Das Gupta, T.Shibata, R.P.Cunninghum and C.M.Radding (1980) The topology of homologous pairing promoted by RecA protein, Cell, 22,437-446
  12. C.M.Radding,T.Shibata,C.K.Das Gupta, RP.Cunninghum, L.Osber (1981) Kinetics and topology of homologous pairing promoted by Escherichia coli RecA protein ( 1981) Cold Spring Hatb Symp Quant.Biol.,45,385-390
  13. RP.Cunninghum, T.Shibata. A.Wu, C.K.Das Gupta and C.M.Radding (1981) Homologous pairing and topological linkage of DNA molecules by combined action of E.coli RecA protein and topoisomerase I ,Cell, 24, 213-223
  14. C.K.Das Gupta, A.Wu, R.Kahn, R.P.Cunninghum and C.M.Radding (1981) Concerted strand exchange and formation of Holliday structure by E.Coli RecA protein, Cell, 25,507-516
  15. T.Shibata, C.K.Das Gupta, R.P.Cunninghum and C.M.Radding (1981) Homologous pairing in genetic recombination, I Biol Chem , 25'6, 7565-7572
  16. C.K.Das Gupta and C.M.Radding (1982) Polar br.mch migration promoted by RecA protein: effect of mismatched base pair, Proc Natl Acad Sci, USA , 79,762-766
  17. C.K.Das Gupta and C.M.Radding ( 19820 Lower fidelity of RecA protein catalysed homologous pairing with a superhelical substrate, Nature, 295, 71- 73
  18. A.Wu, C.K.Das Gupta, RKahn and C.M.Radding (1982) Formation of nascent heteroduplex structures by RecA protein and DNA , Cell, 30,37-44
  19. C.M.Radding, I.Flory, A.Wu, R.Kahn, C.K.Das Gupta, D.Gonda ,M.Bianchi and S.S.Tsang (1983) Three phases of homologous pairing: Polymerization of RecA protein on single stranded DNA, synapsis and polar strand exchange ,Cold Spring Harb Symp Quant BioI, 47,821-828
  20. A. Wu,M.Bianchi, C.K.Das Gupta and C.M.Radding (1983) Unwinding associated with synapsis of DNA molecules by RecA protein, Proc Natl Acad Sci, USA , 80, 1256-1260
  21. RKahn,R.P.Cunninghum,C.K.Das Gupta and C.M.Radding (1981) Polarity of heteroduplex formation promoted by Escherichia coli RecA protein, Proc Natl Acad Sci, USA , 70,4786-4790
  22. M.Bianchi, C.K.Das Gupta and C.M.Radding ( 1983) Synapsis and formation of paranemic joints by E.coli RecA protein, Cell, 34, 931-939
  23. D.Pal and C.Das Gupta (1991) Interaction of some city sewage bacteria with Indian major carp, cirrhinus mrigala, I Aquatic Animal Health, USA , 3,2. 124-129
  24. D.Pal and C.Das Gupta(1992) Microbial pollution in water and its effect on fish, I Aquatic Animal Health, USA, 4, 1, 32-39
  25. D.Pal and C.Das Gupta (1992) Studies on humoral and cellular defence mechanisms of the Indian major carp, Cirrhinus mrigala, Curr Sci, 63, 744-747
  26. B.Das, S.Chattopadhyay and C.Das Gupta (1992) Reactivation of denatured fungal glucose 6- phosphata dehydrogenase and E.coli alkaline phosphatase with E.coli ribosome, Biophys Biochem Res Comm, 183,774-780
  27. B.Das and C.Das Gupta ( 1992) Reconstitution of denatured E.coli alkaline phosphatase with E.coli ribosome, Ind I Biochem Biophys, 29,512-515
  28. A.K.Bera, B.Das, S.Chattopadhyay and C.Das Gupta (1994) Refolding of denatured restriction endonucleases with ribosomal preparations from Methanosarcina barkeri, Biochem Mol Biol Int, 32, 315-323
  29. A.K.Bera, B.Das, S.Chattopadhyay and C.Das Gupta (1994) Protein folding by ribosome and its RNA, Curr Sci, 66, 230-232
  30. S.Chattopadhyay, B.Das, A.K.Bera, D.DasGupta and C.Das Gupta (1994) Refolding of denatured lactate dehydrogeanase by Escherichia coli ribosomes, Biochem J, 300,717-721
  31. A.Chakrabarti, S.Chattopadhyay and C.Das Gupta (1995) Partial unfolding of lactate dehydrogenase in the presence of low concentrations of guanidium chloride, Curr Sci, 68,836-839
  32. B.Das, S.Chattopadhyay, A.K.Bera and C.Das Gupta (1996) In vitro protein folding by ribosomes frq;m Escherichia coli, wheat germ and rat liver: The role of the 50S particle and its 23S rRNA, Eur J Biochem, 235,613-621
  33. S.Chattopadhyay, B.Das and C.Das Gupta (1996) Reactivation of denatured proteins by 23S ribosomal RNA: Role of domain V, Proc Natl Acad Sci, USA , 93,8284-8287
  34. D.Sarkar and C.Das Gupta (1996) Characterizarion of a molten globule intermediate during GdnHcl induced unfolding of RTEM 13 lactamase from Escherichia coli, Biochim et Biophys Acta, 1296, 85-94
  35. D.Pal, S.Chattopadhyay, S.Chandra, D. Sarkar, A. Chakrabarti and C.Das Gupta (1997) Reactivation of denatured proteins by domain V ofbacterial23S rRNA, Nucleic Acid Res, 25, 5047- 5051
  36. S.Chattopadhyay, S.Pal, D.Pal, D.Sarkar, S.Chandra and C.Das Gupta (1999) Protein folding in Escherichia coli: role of23S ribosomal RNA, Biochim et Biophys Acta, 1429,293-298
  37. Chakrabarti,A., Bera, A.K., Das, B., Chattopadhyay, S., Sarkar, D. and Das Gupta, C. (1999) Conformational change of denatured horseradish peroxidase during E.coli ribosome medisted folding, Curr.Sci., 76, 1235-1238.
  38. Das Gupta,C. (1999) Are synthesis and folding of proteins overlapping functions of the ribosomal RNA? , Curr.Sci., 77, 568-573.
  39. Saumen Pal, Suparna Chandra, Saheli Chowdhury, Dibyendu Sarkar, A.N. Ghosh and Chanchal DasGupta. (1999 ) Complementary role of two fragments of Domain V of 23S ribosomal RNA in protein folding. J. Biol. Chem.vol 274,32771-32777.
  40. Saheli Chowdhury, Saumen Pal, Jaydip Ghosh and Chanchal DasGupta Mutations in the Domain V of 23S ribosomal RNA of B.subtilis that inactivate its protein folding property invitro.; 2002, Nucleic Acids Res., vol 30,no. 5, 1278 - 1285.
  41. Suparna Chandra Sanyal, Saumen Pal, Saheli Chaudhuri and Chanchal DasGupta ( 2002) 23S rRNA assisted folding of cytoplasmic malate dehydrogenase is distinctly different from its self folding. Nucleic Acids Res. Vol 30 ( 11 ) , 2390-2397.
  42. Chandra Sanyal,S., Bhattacharya,D. and Das Gupta, C. (2002) The folding of dimeric malate dehydrogenase: Equilibrium and kinetic studies , Eur.J.Biochwm., 269, 3856-3866.
  43. Basu, A., Ghosh, J., Bhattacharya, A., Pal, S., Chowdhuri, S. and Das Gupta, C.(2003) Splitting of ribosome into subunits by unfolded polypeptide chain,., Curr.Sci., 84, 1123-1125.
  44. Ghosh, J., Basu,A., Chowdhuri, S., Bhattacharya, A., Pal, D., Chattoraj, D.K. and Das Gupta, C. (2003) Ribosome-DnaK interaction in relation to protein folding, Mol.Microbiol., 48(6), 1679-1692.
  45. Samanta, D., Mukhopadhyay, D., Chowdhury, S., Ghosh, J., Pal, S., Basu,A., Bhattacharya, A., Das,A., Das, D. and Das Gupta, C. (2008) Protein folding by domain V of Escherichia coli 23S rRNA: specificity of RNA-protein interactions. J Bacteriol., 190, 3344-52.
  46. Basu, A., Samanta, D., Das, D., Chowdhury, S., Bhattacharya, A., Ghosh, J., Das, A., Dasgupta, C. (2008) In vitro protein folding by E. coli ribosome: unfolded protein splitting 70S to interact with 50S subunit. Biochem Biophys Res Commun., 366, 598-603.
  47. Basu, A., Samanta, D., Bhattacharya, A., Das, A., Das, D. and Dasgupta, C. (2008) Protein folding following synthesis in vitro and in vivo: association of newly synthesized protein with 50S subunit of E. coli ribosome. Biochem Biophys Res Commun., 366, 592-597. 5.  Samanta,D., Das,A., Bhattacharya,A., Basu,A., Das, D. And Das Gupta,C.(2009) Mechanism of ribosome assisted protein folding: A new insight into RNA function,  Biochem Biophys Res Commun., 384, 137-140..
  48. Das,D.,Das,A., Samanta,D., Ghosh,J., Dasgupta,S., Bhattacharya,A., Basu,A., Sanyal,S. And Das Gupta,C.(2008) Role of the ribosome in protein folding, Biotechnology journal, 3, 999-1009