Details of LS1102 (Autumn 2012)

Level: 1 Type: Laboratory Credits: 3.0

Course CodeCourse NameInstructor(s)
LS1102 Biology Laboratory I Bidisha Sinha,
Punyasloke Bhadury

Syllabus
SYLLABUS



Module 1: Basic Cell Biology

1. Microscopic observation of prokaryotic and eukaryotic cells (bacteria, blue-gree algae, yeast, plant cells (onion peel), cheek epithelial cells)

2. Plasmolysis and deplasmolysis of onion cells.

3. Staining of blood film and identification of blood cells.



Module 2: Microbiology

1. Media preparation, plating, streaking of bacteria.

2. Gram staining.

3. Antibiotic sensitivity assay.



Module 3: Biochemistry

1. Spectrophotometric estimation of DNA.

2. Bradford's assay for protein concentration.

3. Enzyme kinetics of alkaline phosphatase (varying substrate conc. and varying enzyme conc.)



References

Course Credit Options

Sl. No.ProgrammeSemester NoCourse Choice
1 IP 1 Not Allowed
2 IP 3 Not Allowed
3 MS 1 Core
4 RS 1 Not Allowed