Details of LS2102 (Autumn 2012)
| Level: 2 | Type: Laboratory | Credits: 3.0 |
| Course Code | Course Name | Instructor(s) |
|---|---|---|
| LS2102 | Biology Laboratory III | Chanchal Das Gupta, Mohit Prasad, Rituparna Sinha Roy, Supratim Datta |
| Syllabus |
|---|
| LS2102 : Biology Lab III
SYLLABUS 1. Qualitative test for proteins, ultraviolet absorbance and fluorescence of aromatic amino acids. 2. Qualitative analysis of carbohydrates iodine test for polysaccharide, Fehlings test for reducing sugar, barfoeds test for monosaccharise, Seliwanoffs test for keto sugar and Phenyl Hydrazine Test for Osazone and estimation of Glucose by GOD-POD method. 3. Estimation of vitamin E by Baker and Frank Method 4. Estimation of inorganic phosphate (Fiske and Subbarow method) 5. Estimation of cholesterol content in blood serum 6. Protein labelling by bioconjugation chemistry and quantifying the labelling by UV and fluorescence . 7. Estimation of void volume in Gel Filtration and purification of protein. 8. Protease mediated protein digestion and checking the fragments by Gel Electrophoresis. |
| References |
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Course Credit Options
| Sl. No. | Programme | Semester No | Course Choice |
|---|---|---|---|
| 1 | IP | 1 | Not Allowed |
| 2 | IP | 3 | Not Allowed |
| 3 | MS | 3 | Core |
| 4 | RS | 1 | Not Allowed |