Details of LS1202 (Spring 2016)

Level: 1 Type: Laboratory Credits: 3.0

Course CodeCourse NameInstructor(s)
LS1202 Biology Laboratory II Amirul Islam Mallick,
Malancha Ta,
Rahul Das

Syllabus
Module 1: Basic Cell Biology
1. Microscopic observation of prokaryotic and eukaryotic cells (bacteria, blue-gree algae, yeast, plant cells (onion peel), cheek epithelial cells)
2. Plasmolysis and deplasmolysis of onion cells.
3. Staining of blood film and identification of blood cells.

Module 2: Microbiology
1. Media preparation, plating, streaking of bacteria.
2. Gram staining.
3. Antibiotic sensitivity assay.

Module 3: Biochemistry
1. Spectrophotometric estimation of DNA.
2. Bradford's assay for protein concentration.
3. Enzyme kinetics of alkaline phosphatase (varying substrate conc. and varying enzyme conc.)


References

Course Credit Options

Sl. No.ProgrammeSemester NoCourse Choice
1 IP 2 Not Allowed
2 IP 4 Not Allowed
3 IP 6 Not Allowed
4 MR 2 Not Allowed
5 MR 4 Not Allowed
6 MS 2 Core
7 RS 1 Not Allowed
8 RS 2 Not Allowed