EMBO Practical Course - CEM3DIP 2020: Single particle cryoEM of macromolecular-assemblies and cellular tomography

Partha Pratim Datta, PhD

Associate Professor

Department of Biological Sciences

Indian Institute of Science Education and Research Kolkata

Academic Qualification:
Ph.D. (Molecular Biology), Indian Institute of Chemical Biology, Jadavpur University
M.Sc. (Biotechnology), Madurai Kamaraj University
Positions:
Post-Doctoral Researcher - Wadsworth Center, Albany, NY, USA
Research Scientist - Wadsworth Center, Albany, NY, USA
Assistant Professor - IISER Kolkata
Research Interest:
Studies on the mechanisms of protein synthesis and molecular basis of stress response.
Structural and functional analysis of cellular macromolecular complexes by Cryo-Electron Microscopy, 3D Image processing and molecular biological methods.

Achievement Awards and Honors

Robert J. Colinas Award for Outstanding Postdoctoral Research, Wadsworth Center, NY, USA.

Young Scientist Award, in the 5th International Conference on Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases, Hyderabad, India.

National Eligibility Test Research Fellowship, Council of Scientific and Industrial Research, India.

Junior Fellow Scholarship in Biochemistry, Indian Council of Agricultural Research, India.

National Merit Scholarship, Govt. of India.

Molecular and Structural Biology lab

RESEARCH

Different orientations of the cryo-EM structure of Cucumber Green Mottle Mosaic Virus (CGMMV) reconstructed at 3.5 A

PUBLICATIONS

Chatterjee A, Acharjee A, Das S, Datta PP. Deletion analyses reveal insights into the domain specific activities of an essential GTPase CgtA in Vibrio cholerae. Arch Biochem Biophys. 2019 Apr 15; 665:143-151. doi: 10.1016/j.abb.2019.03.007. Epub 2019 Mar 17.

Hussein M Abkallo, Axel Martinelli,AritaAcharjee, Partha P Datta, Paul Hunt , Richard Carter ,Arnab Pain, Richard Culleton (2017). Rapid Identification of Genes Controlling Virulence and Immunity in Malaria Parasites. Plos Pathogens., 13(7):e1006447.

Chatterjee, A., and Datta, P.P. (2016).Intrinsic GTPase activity of a ribosomal maturation protein CgtA is associated with its inter-domain movement: insights from MD simulations and biochemical studies. J. Biomol. Struct. Dyn.,10.1080/0739110.

Chatterjee, A., and Datta, P.P. (2015). Two conserved amino acids of juxtaposed domains of a ribosomal maturation protein CgtA sustain its optimal GTPase activity. Biochem. Biophys. Res. Commun.,461(4):636-41.

Datta, P.P., and Chatterjee, A. (2013) A passage through the ribosome by Cryo-EM. in Biophysical approaches to translational control of gene expression. Series: Biophysics for the Life Sciences, Vol. 1, Springer, 2013.

Yassin, A.S., Haque, M.E., Datta, P.P., Elmore, K., Banavali, N.K., Spremulli, L.L., Agrawal , R.K. (2011). Insertion domain within mammalian mitochondrial translation initiation factor 2 serves the role of eubacterial initiation factor 1. Proc. Natl. Acad. Sci. (USA), 108(10), 3918-23.

Sharma, M.R., Donhofe, A., Barat, C., Marquez, V., Datta, P.P., Fucini, P., Wilson, D.N., and Agrawal, R.K. (2010). PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the ribosome-recycling factor (RRF) and elongation factor G (EF-G). J. Biol. Chem, 285(6), 4006-4014.

Donhofer, A., Sharma, M.R., Datta, P.P., Nierhaus, K.H., Agrawal, R.K., and Wilson, D.N. (2009). Factor-Mediated Ribosome Assembly in Bacteria: Encyclopedia of Life Sciences, John Wiley & Sons, Ltd: Chichester

Gaur, R, Grasso, D, Datta, P.P., Krishna, P.D.V., Das, G., Spencer, A., Agrawal, R.K., Spremulli, L., and Varshney, U. (2008). A single mammalian mitochondrial translation initiation factor functionally replaces two bacterial factors. Mol. Cell, 29, 180-190. [The structural biological work appeared on the cover-page of theTranslational Control Meeting book, (2008) Cold Spring Harbor Laboratory, NY, USA]

Sharma, M.R., Wilson, D.N., Datta, P.P., Barat, C., Fucini, P., and Agrawal, R.K., (2007). Cryo-EM structure of the Spinaceaoleracea chloroplast 70S ribosome suggests functional roles for plastid-specific ribosomal proteins. Proc. Natl. Acad. Sci. (USA), 104, 19315-19320.

Datta, P.P., Wilson, D.N., Kawazoe, M., Swami, N.K., Sharma, M.R., Booth, T., Shirouzu, M., Takemoto, C.,Fucini, P., and Agrawal, R.K. (2007). Cryo-EM reveals the structural aspects of RbfA function. Mol. Cell, 28, 434-445. [News article, RIKEN Research, Japan]

Barat, C*.,Datta, P.P*., Raj, V.S., Sharma, M.R., Kaji, H., Kaji, A., and Agrawal, R.K. (2007). Progression of the RRF through the ribosome dissociates the ribosomal subunit. Mol. Cell, 27, 250-261. (*co -first authors).

Datta, P.P., Sharma, M.R., Qi, L., Frank, J. and Agrawal, R.K. (2005). Interaction of the G' domain of elongation factor G and the C-terminal domain of ribosomal Protein L7/L12 during translocation, as revealed by cryo-EM. Mol. Cell, 20, 723-731. [Comments on Faculty of 1000]

Palenzuela, L., Hahn, N.M., Nelson Jr., R.P., Arno, J.N., Schobert, C., Bethel, R., Ostrowski, L.A., Sharma, M.R., Datta, P.P., Agrawal, R.K. Schwartz, J. E., and Hirano, M. (2005). Does Linezolid cause lactic acidosis by inhibiting mitochondrial protein synthesis? Clin. Infect. Dis.,40, e113-116.

Datta, P.P., and Bhadra R.K. (2003). Cold shock response and major cold shock proteins of Vibrio cholerae. Appl. Environ. Microbiol.,69, 6361-6369.

Sharma, M.R., Koc, E.C., Datta, P.P., Booth, T., Spremulli, L.L., and Agrawal, R.K. (2003). Structure of the mammalian mitochondrial ribosome reveals an expanded functional role for its component proteins. Cell, 115, 97-108.


  • Studies on the mechanisms of action of an essential ribosome-associated bacterial GTPase protein CgtA.
  • Structural Biological (Cryo-Elecrtron Microscopy) studies on proteins and macro molecular complexes.
    • DBT-RGYI

    DST-SERB

    IISER Kolkata
    1st yr Molecular genetics practical
    2nd yr Molecular Biology theory
    3rd & 4th yr Structural Biology theory
    3rd & 4th yr Structural Biology practical
    4th yr Cell fractionation practical
    5th yr Scientific presentation & communication theory

    • "Indian Institute of Science Education and Research Kolkata (IISER Kolkata) was established in 2006 by the Ministry of Human Resource Development (MHRD), Government of India. IISER Kolkata is designed to reach the prestigious position in the global setting that IISc, IIMs and IITs presently enjoy. IISER Kolkata is an autonomous institution awarding its own degrees. The central theme of the IISER Kolkata is to integrate education with research so that undergraduate teaching as well as doctoral and postdoctoral research work could be carried out in symbiosis."

      About IISER Kolkata

    • "The Department of Biological Sciences at IISER Kolkata integrates cutting edge research with innovative teaching at undergraduate and post-graduate levels, constantly striving towards academic and scientific excellence. Since its inception in 2006, the department has made its mark in biology research and teaching in India. Focusing on the essential unity among biological systems, research in the department spans from the molecular to the organismal levels."

      About Department of Biological Sciences

    • "Research and teaching in the Department of Biological Sciences is supported by state of the art instruments and facilities such as confocal microscopy, flow cytometry, histopathology, tissue culture and facilities for housing and conducting research on various organisms. These are maintained as departmental and institutional facilities, providing easy accessibility to all investigators and allowing collaborative and interdisciplinary research to flourish in the department."

      About Departmental Facilities

    Who we are Meet our team

    ..

    Dr. Partha Pratim Datta

    PI of the Lab

    Dr. Partha Pratim Datta

    PI of the Lab

    Soma Jana

    Senior Research Fellow

    Soma Jana

    Senior Research Fellow

    Sagarika Das

    Senior Research Fellow

    Sagarika Das

    Senior Research Fellow

    Rahul Kumar Singh

    Senior Research Fellow

    Rahul Kumar Singh

    Senior Research Fellow

    ANANYA CHATTERJEE

    Junior Research Fellow

    Ananya Chatterjee

    Junior Research Fellow

    What we do some of our activities

    • Applications are invited for PhD and Post-Doc positions for interested candidates having their own fellowship.
      Please visit IISER Kolkata website and apply through http://apply.iiserkol.ac.in/ that takes formal PhD interview twice a year.
      For rolling interview and relevant information contact partha_datta@iiserkolac.in

      How to apply for the PhD and Post-Doc positions

    Former PhD student(s)

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