Details of LS2103 (Autumn 2013)

Level: 2 Type: Laboratory Credits: 3.0

Course CodeCourse NameInstructor(s)
LS2103 Biology Laboratory III Rituparna Sinha Roy,
Sankar Maiti,
Supratim Datta

Syllabus
1. Qualitative test for proteins, ultraviolet absorbance and fluorescence of aromatic amino acids.
2. Qualitative analysis of carbohydrates iodine test for polysaccharide, Fehlings test for reducing sugar, barfoeds test for monosaccharise, Seliwanoffs test for keto sugar and Phenyl Hydrazine Test for Osazone and estimation of Glucose by GOD-POD method.
3. Estimation of vitamin E by Baker and Frank Method
4. Estimation of inorganic phosphate (Fiske and Subbarow method)
5. Estimation of cholesterol content in blood serum
6. Protein labelling by bioconjugation chemistry and quantifying the labelling by UV and fluorescence .
7. Estimation of void volume in Gel Filtration and purification of protein.
8. Protease mediated protein digestion and checking the fragments by Gel Electrophoresis.

References

Course Credit Options

Sl. No.ProgrammeSemester NoCourse Choice
1 IP 1 Not Allowed
2 IP 3 Not Allowed
3 MS 3 Core
4 RS 1 Not Allowed