Details of LS4202 (Spring 2014)

Level: 4 Type: Theory Credits: 3.0

Course CodeCourse NameInstructor(s)
LS4202 Structural Biology Partha Pratim Datta,
Rituparna Sinha Roy,
Supratim Datta

Syllabus
STRUCTURES\\
Structure of Proteins, Nucleic acids and Membranes: Functional significance of structures.\\
PHYSICAL INSTRUMENTS AND METHODS\\
Diffusion, Sedimentation, Electrophoresis, Separation techniques, Biomolecular structure determination using IR-Raman, UV-visible spectroscopy, CD, ORD, NMR, Fluorescence spectroscopy, Mass spectroscopy and X-ray diffraction.

Basics of Scanning and Transmission Electron Microscopy, Sample preparation, single particle Cryo-Transmission Electron Microscopy, 3D Image processing from EM data, Molecular docking, Model building, computer simulation and graphics, Tomography.

References

  1. Physical Biochemistry : Kensal E van Holde, Curtis Johnson, Pui Shing H
  2. Physical Chemistry with Applications to the Life Sciences : Eisenberg & Crothers (1979).
  3. Molecular Biology of the Cell by Albert et al., (Electron microscopy)
  4. A passage through the ribosome by Cryo-EM, by Partha P. Datta and Ananya Chatterjee. Biophysical approaches to translational control of gene expression. Series: Biophysics for the Life Sciences, Vol. 1, Springer.
  5. SPIDER: http://www.wadsworth.org/spider\_doc/spider/docs/spider.html (Image processing)
  6. EMAN2: http://blake.bcm.edu/emanwiki/EMAN2 (Image processing)
  7. CHIMERA: http://www.cgl.ucsf.edu/chimera/ (Structure analysis)
  8. Additional reading: Three-Dimensional Electron Microscopy ofMacromolecular Assemblies, by Joachim Frank, Oxford university press, 2006.

Course Credit Options

Sl. No.ProgrammeSemester NoCourse Choice
1 IP 2 Not Allowed
2 IP 4 Core
3 MR 2 Not Allowed
4 MR 4 Not Allowed
5 MS 8 Core
6 RS 1 Not Allowed
7 RS 2 Not Allowed