Details of ID4102 (Autumn 2015)

Level: 4 Type: Theory Credits: 3.0

Course CodeCourse NameInstructor(s)
ID4102 Fluorescence Spectroscopy: Principle and Applications Prasun Kumar Mandal

Syllabus
1. Introduction: Electronic Spectroscopy and Jablonski Diagram

2. Instrumentation

3. Fluorophores

4. Spectral and Time-Resolved Fluorescence

5. Solvent and Environmental Effects on Fluorescence

6. Dynamics of Solvent and Spectral Relaxation

7. Fluorescence Anisotropy: Spectral and Temporal Aspects

8. Resonance Energy Transfer: Spectral and Temporal Aspects

9. Fluorescence Correlation Spectroscopy

10. Protein Fluorescence

11. Fluorescence Sensing

12. Novel Fluorophores: Quantum Dots etc.

13. Multiphoton Fluorescence Spectroscopy and Microscopy

14. Fluorescence Lifetime Imaging Microscopy

15. Single Molecule Fluorescence Spectroscopy and Microscopy

References
1. Fluorescence Spectroscopy, by J. R. Lakowicz, Springer, (2006).

2. Molecular Fluorescence - Principles and Applications by B. Valeur, Wiley-VCH, (2002).

Course Credit Options

Sl. No.ProgrammeSemester NoCourse Choice
1 IP 1 Not Allowed
2 IP 3 Elective
3 IP 5 Not Allowed
4 MR 1 Not Allowed
5 MR 3 Not Allowed
6 MS 7 Elective
7 RS 1 Elective
8 RS 2 Elective