Details of LS3205 (Spring 2017)

Level: 3 Type: Laboratory Credits: 3.0

Course CodeCourse NameInstructor(s)
LS3205 Biology Laboratory-VIII Jayasri Das Sarma,
Rupak Datta

This lab based course introduces modern tools of molecular cloning; protein expression and purification and is designed for upper level undergraduate students majoring in Biology. Students learn how to apply a range of recombinant DNA tools to modern biochemical research.

Cloning and Protein Expression Laboratory

  • Cloning of DNA fragments:Cloning of gene by PCR method; Restriction Digestion of the gene; Ligation and Transformation of the gene into a plasmid; Plasmid DNA preparation; Verification of isolated plasmid DNA;

  • Expression of protein:Preparation of competent cells; Transformation of clone into competent cells; Growth and induction with IPTG; Harvesting and preparation of cell lysate; SDS-Gel electrophoresis;


Course Credit Options

Sl. No.ProgrammeSemester NoCourse Choice
1 IP 2 Elective
2 IP 4 Elective
3 IP 6 Not Allowed
4 MR 2 Not Allowed
5 MR 4 Not Allowed
6 MS 6 Core
7 RS 1 Not Allowed
8 RS 2 Not Allowed