Details of ID4102 (Autumn 2018)

Level: 4 Type: Theory Credits: 4.0

Course CodeCourse NameInstructor(s)
ID4102 Fluorescence Spectroscopy: Principles and Applications Prasun Kumar Mandal

Syllabus
1. Introduction: Electronic Spectroscopy and Jablonski Diagram
2. Instrumentation
3. Fluorophores
4. Spectral and Time-Resolved Fluorescence
5. Solvent and Environmental Effects on Fluorescence
6. Dynamics of Solvent and Spectral Relaxation
7. Fluorescence Anisotropy: Spectral and Temporal Aspects
8. Resonance Energy Transfer: Spectral and Temporal Aspects
9. Fluorescence Correlation Spectroscopy
10. Protein Fluorescence
11. Fluorescence Sensing
12. Novel Fluorophores: Quantum Dots etc.
13. Multiphoton Fluorescence Spectroscopy and Microscopy
14. Fluorescence Lifetime Imaging Microscopy
15. Single Molecule Fluorescence Spectroscopy and Microscopy

References
1. Fluorescence Spectroscopy, by J. R. Lakowicz, Springer, (2006).
2. Molecular Fluorescence - Principles and Applications by B. Valeur, Wiley-VCH, (2002).

Course Credit Options

Sl. No.ProgrammeSemester NoCourse Choice
1 IP 1 Not Allowed
2 IP 3 Elective
3 IP 5 Elective
4 MR 1 Not Allowed
5 MR 3 Not Allowed
6 MS 7 Elective
7 RS 1 Elective
8 RS 2 Elective