Details of LS3104 (Autumn 2019)

Level: 3 Type: Laboratory Credits: 4.0

Course CodeCourse NameInstructor(s)
LS3104 Biology Laboratory V Bidisha Sinha,
Dipjyoti Das,
Sankar Maiti

Tissue Culture, Immunofluorescence, Basic Fluorescence Microscopy and Image Analysis


  • Tissue Culture: Media Preparation, Trypsinization and Culturing eukaryotic cells; Hemocytometer counting of cells; Cryo-preservation of cells;

  • Immunofluorescence: Using Primary and fluorescently labelled secondary antibody to localize protein distribution in cells;

  • Basic Fluorescence Microscopy: Measuring Point Spread Function, Resolution, Scale Calibration, camera-based Image acquisition;

  • Image Analysis: Basic Image Analysis with FiJi/Matlab Understanding Digital Images; Object Detection; Image registration; Introduction to coding for Image analysis

  • Short project: Design an imaging-based short experiment and use image analysis tools to extract information.


Course Credit Options

Sl. No.ProgrammeSemester NoCourse Choice
1 IP 1 Elective
2 IP 3 Not Allowed
3 IP 5 Not Allowed
4 MR 1 Not Allowed
5 MR 3 Not Allowed
6 MS 5 Core
7 RS 1 Not Allowed
8 RS 2 Not Allowed