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Details of LS3205 (Spring 2020)

Level: 3 Type: Laboratory Credits: 4.0

Course CodeCourse NameInstructor(s)
LS3205 Cloning and Protein Expression Laboratory Jayasri Das Sarma,
Rupak Datta

Preamble
Cloning and Protein Expression Laboratory



  • Cloning of DNA fragments:Cloning of gene by PCR method; Restriction Digestion of the gene; Ligation and Transformation of the gene into a plasmid; Plasmid DNA preparation; Verification of isolated plasmid DNA;

  • Expression of protein:Preparation of competent cells; Transformation of clone into competent cells; Growth and induction with IPTG; Harvesting and preparation of cell lysate; SDS-Gel electrophoresis;




Syllabus
Cloning and Protein Expression Laboratory



  • Cloning of DNA fragments:Cloning of gene by PCR method; Restriction Digestion of the gene; Ligation and Transformation of the gene into a plasmid; Plasmid DNA preparation; Verification of isolated plasmid DNA;

  • Expression of protein:Preparation of competent cells; Transformation of clone into competent cells; Growth and induction with IPTG; Harvesting and preparation of cell lysate; SDS-Gel electrophoresis;




References

Course Credit Options

Sl. No.ProgrammeSemester NoCourse Choice
1 IP 2 Elective
2 IP 4 Not Allowed
3 IP 6 Not Allowed
4 MR 2 Not Allowed
5 MR 4 Not Allowed
6 MS 6 Core
7 RS 1 Not Allowed
8 RS 2 Not Allowed

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