Details of LS4201 (Spring 2020)

Level: 4 Type: Theory Credits: 4.0

Course CodeCourse NameInstructor(s)
LS4201 Structural Biology Partha Pratim Datta,
Rituparna Sinha Roy


  • Peptide, proteins and Nucleic acids conformation: Organization of secondary structural elements of proteins; Structural motifs and analysis of informations from protein data bank; Origin and importance of Ramachandran Plot; Introductory structural bioinformatics; Protein folding, misfolding and diseases;

    Biomolecular structure determination using UV-Vis, IR, Electronic Circular Dichroism, Vibrational Dichroism, Dynamic Light Scattering, Fluorescence, Mass Spectroscopy and proteomics tools, Basic one dimensional and Two Dimensional NMR Spectroscopy

  • : X-ray diffraction and Cryo-Eelectron Microscopy: Basics of Scanning and Transmission Electron Microscopy, Sample preparation, single particle Cryo-Transmission Electron Microscopy, 3D Image processing from EM data, Model building and Molecular docking, Tomography;


  1. Ramachandran, G.N., and Sasisekharan, V. Advances in Protein Chemistry, Vol. 23, Academic Press, P. 283 (1968).
  2. Physical Biochemistry: Kensal E van Holde, Curtis Johnson, PuiShing H
  3. Principles of Protein Structure: Schulz and Schirmer (1979)
  4. NMR of proteins and nucleic acids: K. Whtrich (1986).
  5. Molecular Biology of the Cell by Albert et al. (Electron microscopy)
  6. A passage through the ribosome by Cryo-EM, by Partha P. Datta and Ananya Chatterjee. Biophysical approaches to translational control of gene expression. Series: Biophysics for the Life Sciences, Vol. 1, Springer.
  7. SPIDER:\_doc/spider/docs/spider.html (Image processing)
  8. EMAN2: (Image processing)
  9. RELION:
  10. CHIMERA: (Structure analysis)
  11. Additional reading: Three-Dimensional Electron Microscopy of Macromolecular Assemblies, by Joachim Frank, Oxford university press, 2006.

Course Credit Options

Sl. No.ProgrammeSemester NoCourse Choice
1 IP 2 Not Allowed
2 IP 4 Core
3 IP 6 Not Allowed
4 MR 2 Not Allowed
5 MR 4 Not Allowed
6 MS 8 Core
7 RS 1 Elective
8 RS 2 Elective