Details of CH4106 (Autumn 2023)

Level: 4 Type: Theory Credits: 4.0

Course CodeCourse NameInstructor(s)
CH4106 Fluorescence Spectroscopy: Principles and Applications Prasun Kumar Mandal

Syllabus
1. Introduction: Electronic Spectroscopy and Jablonski Diagram (2 Lecture)
2. Instrumentation (4 Lecture)
3. Fluorophores (4 Lecture)
4. Spectral and Time-Resolved Fluorescence (2 Lecture)
5. Solvent and Environmental Effects on Fluorescence (2 Lecture)
6. Fluorescence Quenching (2 Lecture)
7. Dynamics of Solvent and Spectral Relaxation (2 Lecture)
8. Fluorescence Anisotropy: Spectral and Temporal Aspects (4 Lecture)
9. Resonance Energy Transfer: Spectral and Temporal Aspects (4 Lecture)
10. Fluorescence Correlation Spectroscopy (3 Lecture)
11. Protein Fluorescence (2 Lecture)
12. Fluorescence Sensing (3 Lecture)
13. Novel Fluorophores: Quantum Dots etc. (2 Lecture)
14. Multiphoton Fluorescence Spectroscopy and Microscopy (2 Lecture)
15. Fluorescence Lifetime Imaging Microscopy (1 Lecture)
16. Single Molecule Fluorescence Spectroscopy and Microscopy (1 Lecture)

References
1. Fluorescence Spectroscopy, by J. R. Lakowicz, Springer, (2006).
2. Molecular Fluorescence - Principles and Applications by B. Valeur, Wiley-VCH, (2002).

Course Credit Options

Sl. No.ProgrammeSemester NoCourse Choice
1 IP 1 Elective
2 IP 3 Elective
3 IP 5 Not Allowed
4 MP 1 Not Allowed
5 MP 3 Elective
6 MR 1 Elective
7 MR 3 Not Allowed
8 MS 3 Not Allowed
9 MS 5 Not Allowed
10 MS 7 Elective
11 MS 9 Elective
12 RS 1 Elective
13 RS 2 Elective