Details of LS3205 (Spring 2023)
| Level: 3 | Type: Laboratory | Credits: 4.0 |
| Course Code | Course Name | Instructor(s) |
|---|---|---|
| LS3205 | Cloning and Protein Expression Laboratory | Babu Sudhamalla, Jayasri Das Sarma |
| Preamble |
|---|
| This lab-based course introduces modern tools of molecular cloning; protein expression and
purification and is designed for upper level undergraduate students majoring in Biology. Students learn how to apply a range of recombinant DNA tools to modern biochemical research. |
| Syllabus |
|---|
| Cloning and Protein Expression Laboratory
Cloning of DNA fragments: Cloning of gene by PCR method; Restriction Digestion of the gene; Ligation and Transformation of the gene into a plasmid; Plasmid DNA preparation; Verification of isolated plasmid DNA; Expression of protein: Preparation of competent cells; Transformation of clone into competent cells; Growth and induction with IPTG; Harvesting and preparation of cell lysate; SDS-Gel electrophoresis |
| References |
|---|
Course Credit Options
| Sl. No. | Programme | Semester No | Course Choice |
|---|---|---|---|
| 1 | IP | 2 | Elective |
| 2 | IP | 4 | Elective |
| 3 | IP | 6 | Not Allowed |
| 4 | MP | 2 | Not Allowed |
| 5 | MP | 4 | Not Allowed |
| 6 | MR | 2 | Not Allowed |
| 7 | MR | 4 | Not Allowed |
| 8 | MS | 10 | Elective |
| 9 | MS | 4 | Not Allowed |
| 10 | MS | 6 | Core |
| 11 | MS | 8 | Elective |
| 12 | RS | 1 | Elective |
| 13 | RS | 2 | Elective |