Details of CH4106 (Autumn 2025)
| Level: 4 | Type: Theory | Credits: 4.0 |
| Course Code | Course Name | Instructor(s) |
|---|---|---|
| CH4106 | Fluorescence Spectroscopy: Principles and Applications | Prasun Kumar Mandal |
| Syllabus |
|---|
| 1. Introduction: Electronic Spectroscopy and Jablonski Diagram (2 Lecture)
2. Instrumentation (4 Lecture) 3. Fluorophores (4 Lecture) 4. Spectral and Time-Resolved Fluorescence (2 Lecture) 5. Solvent and Environmental Effects on Fluorescence (2 Lecture) 6. Fluorescence Quenching (2 Lecture) 7. Dynamics of Solvent and Spectral Relaxation (2 Lecture) 8. Fluorescence Anisotropy: Spectral and Temporal Aspects (4 Lecture) 9. Resonance Energy Transfer: Spectral and Temporal Aspects (4 Lecture) 10. Fluorescence Correlation Spectroscopy (3 Lecture) 11. Protein Fluorescence (2 Lecture) 12. Fluorescence Sensing (3 Lecture) 13. Novel Fluorophores: Quantum Dots etc. (2 Lecture) 14. Multiphoton Fluorescence Spectroscopy and Microscopy (2 Lecture) 15. Fluorescence Lifetime Imaging Microscopy (1 Lecture) 16. Single Molecule Fluorescence Spectroscopy and Microscopy (1 Lecture) |
| References |
|---|
| 1. Fluorescence Spectroscopy, by J. R. Lakowicz, Springer, (2006).
2. Molecular Fluorescence - Principles and Applications by B. Valeur, Wiley-VCH, (2002). |
Course Credit Options
| Sl. No. | Programme | Semester No | Course Choice |
|---|---|---|---|
| 1 | IP | 1 | Not Allowed |
| 2 | IP | 3 | Not Allowed |
| 3 | IP | 5 | Not Allowed |
| 4 | MP | 1 | Not Allowed |
| 5 | MP | 3 | Elective |
| 6 | MR | 1 | Elective |
| 7 | MR | 3 | Not Allowed |
| 8 | MS | 3 | Not Allowed |
| 9 | MS | 5 | Not Allowed |
| 10 | MS | 7 | Elective |
| 11 | MS | 9 | Elective |
| 12 | RS | 1 | Elective |
| 13 | RS | 2 | Elective |