- Tissue Culture: Media Preparation, Trypsinization and Culturing eukaryotic cells; Hemocytometer counting of cells; Cryo-preservation of cells;
- Immunofluorescence: Using Primary and fluorescently labelled secondary antibody to localize protein distribution in cells;
- Basic Fluorescence Microscopy: Measuring Point Spread Function, Resolution, Scale Calibration, camera-based Image acquisition;
- Image Analysis: Basic Image Analysis with FiJi/Matlab Understanding Digital Images; Object Detection; Image registration; Introduction to coding for Image analysis
- Short project: Design an imaging-based short experiment and use image analysis tools to extract information.
- Basic Flow cytometry: Basic introduction to Flow Cytometer. Understanding the principles and working of a Flow Cytometer. Designing a simple experiment that will demonstrate how two lineages of cells can be counted and separated in a Flow Cytometer.
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