Details of LS3203 (Spring 2025)
| Level: 3 | Type: Theory | Credits: 4.0 |
| Course Code | Course Name | Instructor(s) |
|---|---|---|
| LS3203 | Structural Biology | Partha Pratim Datta, Rituparna Sinha Roy |
| Syllabus |
|---|
| Peptide, proteins and Nucleic acids conformation: Organization of secondary structural
elements of proteins; Structural motifs and analysis of informations from protein data bank; Origin and importance of Ramachandran Plot; Introductory structural bioinformatics; Protein folding, misfolding and diseases; Physical Instruments And Methods: Biomolecular structure determination using UV-Vis, IR, Electronic Circular Dichroism, Vibrational Dichroism, Dynamic Light Scattering, Fluorescence, Mass Spectroscopy and proteomics tools, Basic one dimensional and Two Dimensional NMR Spectroscopy X-ray diffraction and Cryo-Eelectron Microscopy: Basics of Scanning and Transmission Electron Microscopy, Sample preparation, single particle Cryo-Transmission Electron Microscopy, 3D Image processing from EM data, Model building and Molecular docking, Tomography. |
| References |
|---|
| 1. Ramachandran, G.N., and Sasisekharan, V. Advances in Protein Chemistry, Vol. 23,
Academic Press, P. 283 (1968). 2. Physical Biochemistry: Kensal E van Holde, Curtis Johnson, PuiShing H 3. Principles of Protein Structure: Schulz and Schirmer (1979) 4. NMR of proteins and nucleic acids: K. Whtrich (1986). 5. Molecular Biology of the Cell by Albert et al. (Electron microscopy) 6. A passage through the ribosome by Cryo-EM, by Partha P. Datta and Ananya Chatterjee. Biophysical approaches to translational control of gene expression. Series: Biophysics for the Life Sciences, Vol. 1, Springer. 7. SPIDER: http://www.wadsworth.org/spider\_doc/spider/docs/spider.html (Image processing) 8. EMAN2: http://blake.bcm.edu/emanwiki/EMAN2 (Image processing) 9. RELION: http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page 10. CHIMERA: http://www.cgl.ucsf.edu/chimera/ (Structure analysis) 11. Additional reading: Three-Dimensional Electron Microscopy of Macromolecular Assemblies, by Joachim Frank, Oxford university press, 2006. |
Course Credit Options
| Sl. No. | Programme | Semester No | Course Choice |
|---|---|---|---|
| 1 | IP | 2 | Elective |
| 2 | IP | 4 | Elective |
| 3 | IP | 6 | Not Allowed |
| 4 | MP | 2 | Not Allowed |
| 5 | MP | 4 | Elective |
| 6 | MR | 2 | Not Allowed |
| 7 | MR | 4 | Not Allowed |
| 8 | MS | 10 | Elective |
| 9 | MS | 4 | Not Allowed |
| 10 | MS | 6 | Core |
| 11 | MS | 8 | Elective |
| 12 | RS | 1 | Elective |
| 13 | RS | 2 | Elective |