Details of LS3104 (Autumn 2026)
| Level: 3 | Type: Laboratory | Credits: 4.0 |
| Course Code | Course Name | Instructor(s) |
|---|---|---|
| LS3104 | Cell Biology and Imaging Laboratory | Arnab Gupta, Sankar Maiti |
| Syllabus |
|---|
| Syllabus
Tissue Culture: Media Preparation, Trypsinization and Culturing eukaryotic cells; Hemocytometer counting of cells; Cryo-preservation of cells; Immunofluorescence: Using Primary and fluorescently-labelled secondary antibody to localize protein distribution in cells; Basic Fluorescence Microscopy: Measuring Point Spread Function, Resolution, Scale Calibration, camera-based Image acquisition; Image Analysis: Basic Image Analysis with FiJi/Matlab Understanding Digital Images; Object Detection; Image registration; Introduction to coding for Image analysis Short project: Design an imaging-based short experiment and use image analysis tools to extract information. Basic Flow cytometry: Basic introduction to Flow Cytometer. Understanding the principles and working of a Flow Cytometer. Designing a simple experiment that will demonstrate how two lineages of cells can be counted and separated in a Flow Cytometer. |
| References |
|---|
| References
Study material will be provided by the instructors as required. |
Course Credit Options
| Sl. No. | Programme | Semester No | Course Choice |
|---|---|---|---|
| 1 | IP | 1 | Elective |
| 2 | IP | 3 | Not Allowed |
| 3 | MP | 1 | Not Allowed |
| 4 | MP | 3 | Not Allowed |
| 5 | MR | 1 | Elective |
| 6 | MR | 3 | Not Allowed |
| 7 | MS | 3 | Not Allowed |
| 8 | MS ( Biological Sciences ) | 5 | Core |
| 9 | MS | 7 | Elective |
| 10 | MS | 9 | Elective |
| 11 | RS | 1 | Not Allowed |
| 12 | RS | 2 | Not Allowed |