Details of LS3105 (Autumn 2026)
| Level: 3 | Type: Laboratory | Credits: 4.0 |
| Course Code | Course Name | Instructor(s) |
|---|---|---|
| LS3105 | Gene Expression Laboratory | Malancha Ta, Partha Pratim Datta, Sreeramaiah N Gangappa |
| Preamble |
|---|
| This course typically focuses on the techniques and methods used to study gene activity in cells. Students learn how to analyze mRNA levels, perform PCR, Northern blotting, qPCR, and RNA sequencing, and explore how gene expression is regulated in response to various stimuli. The course often includes hands-on experience with laboratory equipment, data analysis, and interpretation of results, providing a deep understanding of how genes are turned on or off in different biological contexts. |
| Syllabus |
|---|
| Syllabus
Detection of proteins by SDS-PAGE followed by Western blot detection and analysis Polymerase Chain Reaction (PCR), Semi-quantitative and Quantitative PCR analysis: Principle and Methods of PCR, Types of PCR and specific applications. Understanding the functions of various components used in the PCR reaction. Different types of polymerases and their specific features. Difference between Semi-quantitative and Quantitative PCR analysis. Principle of qPCR- multiple chemistries such as SYBR-Green, TaqMan, Molecular Beacons, etc., their specific advantages. Applications of qPCR in genetics, genomics, agriculture and Biomedical Sciences. ELISA: Principle, methodology and application, different types of ELISA, detection of Antigen/Antibody concentration of test samples. |
| References |
|---|
| References
Study material will be provided by the instructors as required. |
Course Credit Options
| Sl. No. | Programme | Semester No | Course Choice |
|---|---|---|---|
| 1 | IP | 1 | Elective |
| 2 | IP | 3 | Not Allowed |
| 3 | MP | 1 | Not Allowed |
| 4 | MP | 3 | Not Allowed |
| 5 | MR | 1 | Elective |
| 6 | MR | 3 | Not Allowed |
| 7 | MS | 3 | Not Allowed |
| 8 | MS ( Biological Sciences ) | 5 | Core |
| 9 | MS | 7 | Elective |
| 10 | MS | 9 | Elective |
| 11 | RS | 1 | Not Allowed |
| 12 | RS | 2 | Not Allowed |