Details of LS4114 (Autumn 2026)
| Level: 4 | Type: Laboratory | Credits: 4.0 |
| Course Code | Course Name | Instructor(s) |
|---|---|---|
| LS4114 | Chemical Biology Laboratory I | Rahul Das, Supratim Datta |
| Preamble |
|---|
| The Molecules of Life: Physical and Chemical Principles by John Kuriyan, Boyana
Konforti, and David Wemmer, Biochemistry by Voet and Voet, Lewins Gene XII, Essentials of Chemical Biology. Structure and Dynamics of Biological Macromolecules Andrew Miller and Julian Tanner. Introduction to Bioorganic Chemistry and Chemical Biology. David Van Vranken and Gregory Weiss, Foundations of Chemical Biology. C. M. I. Dobson J. A. Gerrard, and A. J. Pratt |
| Syllabus |
|---|
| Characterization of Amino acids by TLC
DNA and Protein estimation Bradford and UV DNA Unfolding by UV-vis Protein Folding by CD spectroscopy Recombinant DNA technology - Plasmid Purification and Restriction Enzyme Digestion - Agarose Gel Electrophoresis Techniques in protein expression and purification - Growth curve (Secondary culture with and without induction) - His tag purification with beads and preparation of the SDS-PAGE sample - Antibody purification using Protein-A column Measuring interactions - ITC - Binding Kinetics by Stopped-flow - SPR |
| References |
|---|
| Study materials will be provided in class. |
Course Credit Options
| Sl. No. | Programme | Semester No | Course Choice |
|---|---|---|---|
| 1 | IP | 1 | Not Allowed |
| 2 | IP | 3 | Not Allowed |
| 3 | MP | 1 | Not Allowed |
| 4 | MP ( Chemical Biology ) | 1 | Core |
| 5 | MP | 3 | Not Allowed |
| 6 | MR | 1 | Not Allowed |
| 7 | MR | 3 | Not Allowed |
| 8 | MS | 3 | Not Allowed |
| 9 | MS | 5 | Not Allowed |
| 10 | MS | 7 | Not Allowed |
| 11 | MS | 9 | Not Allowed |
| 12 | RS | 1 | Not Allowed |
| 13 | RS | 2 | Not Allowed |