Details of LS3203 (Spring 2026)
| Level: 3 | Type: Theory | Credits: 4.0 |
| Course Code | Course Name | Instructor(s) |
|---|---|---|
| LS3203 | Structural Biology | Partha Pratim Datta, Rituparna Sinha Roy |
| Preamble |
|---|
| This course gives an overview about advanced structural features of peptide and protein structural biology, protein folding, structure determination related techniques and principles of developing protein based therapeutics. Furthermore, this course covers details of the principles of determining 2D and 3D structures of biological objects at various levels of sizes and resolutions and discusses their applications and usages. |
| Syllabus |
|---|
| Syllabus
Detailed information about peptide and protein structure, Detailed information about Ramachandran map, Peptide designing as putative molecular medicine for disease biology and protein folding and misfolding. Biophysical Techniques like Circular Dichroism, Vibrational Circular Dichroism, Basic 1D and 2D NMR spectroscopy of peptides, introductory mass spectrometry, and introductory fluorescence An overview of the visualizing and understanding of the 2D and 3D structures of biological objects at various levels. Principles of Scanning Electron Microscopy and its Applications. Principles of Transmission Electron Microscopy and its Applications. Principles of Single Particle Cryo-Electron Microscopy (Cryo-EM) and its applications. Principles of Cryo-Electron Tomography (Cryo-ET) and its applications. Principles of X-Ray crystallography and its applications. Principles of Small Angle X-Ray Scattering (SAXS) and its applications. |
| References |
|---|
| References:
1. Ramachandran, G.N., and Sasisekharan, V. Advances in Protein Chemistry, Vol. 23, Academic Press, P. 283 (1968). 2. Physical Biochemistry: Kensal E van Holde, Curtis Johnson, PuiShing H 3. Principles of Protein Structure: Schulz and Schirmer (1979) 4. NMR of proteins and nucleic acids: K. Whtrich (1986). 5. Molecular Biology of the Cell by Albert et al. (Electron microscopy) 6. A passage through the ribosome by Cryo-EM, by Partha P. Datta and Ananya Chatterjee. Biophysical approaches to translational control of gene expression. Series: Biophysics for the Life Sciences, Vol. 1, Springer 7. SPIDER: http://www.wadsworth.org/spider\_doc/spider/docs/spider.html (Image processing) 8. EMAN2: http://blake.bcm.edu/emanwiki/EMAN2 (Image processing) 9. RELION: http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page 10. CHIMERA: http://www.cgl.ucsf.edu/chimera/ (Structure analysis) 11. Additional reading: Three-Dimensional Electron Microscopy of Macromolecular Assemblies, by Joachim Frank, Oxford university press, 2006. |
Course Credit Options
| Sl. No. | Programme | Semester No | Course Choice |
|---|---|---|---|
| 1 | IP | 2 | Elective |
| 2 | IP | 4 | Elective |
| 3 | MP | 2 | Elective |
| 4 | MP | 2 | Not Allowed |
| 5 | MP | 4 | Not Allowed |
| 6 | MR | 2 | Elective |
| 7 | MR | 4 | Elective |
| 8 | MS | 10 | Elective |
| 9 | MS | 4 | Not Allowed |
| 10 | MS ( Biological Sciences ) | 6 | Core |
| 11 | MS | 8 | Elective |
| 12 | RS | 1 | Elective |
| 13 | RS | 2 | Elective |