Details of LS4201 (Spring 2026)
| Level: 4 | Type: Laboratory | Credits: 4.0 |
| Course Code | Course Name | Instructor(s) |
|---|---|---|
| LS4201 | Biochemistry and Structural Biology Laboratory | Partha Pratim Datta, Rahul Das, Rituparna Sinha Roy, Supratim Datta |
| Preamble |
|---|
| The course trains students to methods for characterization of proteins through experiments and data analysis. |
| Syllabus |
|---|
| Syllabus
Biophysical properties of enzymes through spectroscopy and kinetics: Effect of inhibitors on enzyme kinetics First-order binding between calcium and EDTA using ITC Measuring kinetic component of structural rearrangement in Abl and imatinib interaction by using stopped-flow fluorescence spectroscopy Protein structural analysis, sequence alignment, structure prediction, model building, and different software related to structural biology Demonstration and data analysis related to protein secondary structure by Circular Dichroism spectroscopy Peptide NMR analysis 1D and 2D Fluorescence of Tryptophan in polar and non-polar environment HPLC demonstration and data analysis Demonstration and data analysis by SEM and TEM. |
| References |
|---|
| References
Reading material will be provided by instructors. |
Course Credit Options
| Sl. No. | Programme | Semester No | Course Choice |
|---|---|---|---|
| 1 | IP | 2 | Elective |
| 2 | IP | 4 | Elective |
| 3 | MP | 2 | Not Allowed |
| 4 | MP | 4 | Not Allowed |
| 5 | MR | 2 | Elective |
| 6 | MR | 4 | Elective |
| 7 | MS | 10 | Elective |
| 8 | MS | 4 | Not Allowed |
| 9 | MS | 6 | Not Allowed |
| 10 | MS ( Biological Sciences ) | 8 | Core |
| 11 | RS | 1 | Elective |
| 12 | RS | 2 | Elective |