Details of LS4203 (Spring 2026)
| Level: 4 | Type: Theory | Credits: 4.0 |
| Course Code | Course Name | Instructor(s) |
|---|---|---|
| LS4203 | Gene Regulation | Arnab Gupta, Sumit Sen Santara |
| Preamble |
|---|
| Gene regulation governs how cells control gene expression in response to internal and external signals. This course explores key regulatory mechanisms in prokaryotic and eukaryotic systems at transcriptional, post-transcriptional, translational, and post-translational levels. It also discusses gene regulation in cellular communication, signal transduction, and stress responses, including nutrient regulation, mTORC signaling, lysosomal function, and oxidative stress management. |
| Syllabus |
|---|
| Syllabus
Basic concepts in prokaryotic gene regulation: Components of prokaryotic gene regulation Constitutive, Inducible, and Repressible Gene Expression; Fine regulation (operon concept: lac, Trp and lambda operons as models), Translational and post translational regulatory mechanism. Basic concepts in eukaryotic gene regulation: Eukaryotic transcriptional control (RNA polymerases, transcription factors), post-transcriptional, processes (splicing, mRNA export, localization, translation, decay, siRNA, miRNA), post-translational modifications as means of regulation of gene function. Chromatin remodelling and Epigenetic regulation. Regulation of viral gene expression. Gene regulation in cellular communication: Response to stimuli as a determinant of gene regulation, ligand-receptor interactions, calculation of dissociation constants and Scatchard analysis, nature and variety of signal transduction pathways. Regulatory networks and allosteric regulation of cellular signaling, Regulation of GPCRs. Gene regulation in stress response: Nutrient-mediated regulation of gene expression, Cellular response in stress, Nutrient distribution in the cell, mTORC mediated regulation of amino acids and other micronutrients, lysosomes in nutrient recycling and translation regulation, lysosomal positioning as a read-out of cellular stress status, mTOR and cancer, Superoxide dismutase as a scavenger of oxidative stress. |
| References |
|---|
| References
1. Molecular Biology of the Cell Bruce Alberts et al., W.W. Norton & Company, Edition: 7th (2022) 2. Molecular Biology- David P. Clark, Academic Cell, 3rd Edition (2018) 3. Gene Cloning and DNA Analysis: An Introduction T.A. Brown, Wiley-Blackwell, 7th Edition (2016) 4. Molecular Biology of the Gene- James D. Watson, along with Tania A. Baker, Pearson, 7th Edition (2013) 5. Concepts of Genetics- William S. Klug, Michael R. Cummings, Pearson Edition: 12th (2018) 6. Molecular Genetics of Bacteria Larry Snyder & Wendy Champness, ASM Press, Edition: 4th (2013) |
Course Credit Options
| Sl. No. | Programme | Semester No | Course Choice |
|---|---|---|---|
| 1 | IP | 2 | Elective |
| 2 | IP | 4 | Elective |
| 3 | MP | 2 | Not Allowed |
| 4 | MP | 4 | Not Allowed |
| 5 | MR | 2 | Elective |
| 6 | MR | 4 | Elective |
| 7 | MS | 10 | Elective |
| 8 | MS | 4 | Not Allowed |
| 9 | MS | 6 | Not Allowed |
| 10 | MS | 8 | Elective |
| 11 | RS | 1 | Elective |
| 12 | RS | 2 | Elective |