Details of LS4209 (Spring 2026)
| Level: 4 | Type: Theory | Credits: 4.0 |
| Course Code | Course Name | Instructor(s) |
|---|---|---|
| LS4209 | Mass Spectrometry and its Application in Molecular Medicine | Amit Kumar Mandal |
| Preamble |
|---|
| Mass spectrometry is an analytical platform that measures mass of molecules. Two soft-ionization techniques, ESI-MS and MALDI-MS are used to visualize large biomolecules. LC/ESI-MS can be used to quantify oxidative stress, characterization of hemoglobinopathies etc. Nano-LC/ESI-MS and 2D gel in combination with MALDI-MS platforms are used to discover disease biomarkers. Using native mass spectrometry, it is possible to analyze noncovalently bound biological complexes. Hydrogen/Deuterium exchange mass spectrometry provides conformational details of proteins associated with physiological/biochemical events. Imaging mass spectrometry provides the molecular portrait of the heterogeneous distribution of proteins and metabolites across a tissue section. |
| Syllabus |
|---|
| Syllabus
Basics of mass spectrometry Electrospray ionization mass spectrometry (ESI-MS) Matrix Assisted Laser Desorption Ionisation mass spectrometry (MALDI-MS) Tandem mass spectrometry and sequencing of peptides/protein LC-based proteomics D/2D Gel-based proteomics Quantitative proteomics Isotope exchange based mass spectrometry and its application Native mass spectrometry and its application Ion mobility mass spectrometry Imaging mass spectrometry and tissue imaging |
| References |
|---|
| References
1. Protein Sequencing and Identification Using Tandem Mass Spectrometry: Michael Kinter, Nicholas E. Sherman 2. Mass spectrometry: Principles and Application. Edmond de Hoffmann, Vincent Stroobant 3. Proteomics Today. Mahmoud Hamdan, Pier Giorgio Righetti 4. Biological Mass Spectrometry, Methods in Enzymology, Volume 402 5. Hydrogen Exchange Mass Spectrometry of Proteins: Fundamental, Methods, and Applications. David D. Weis 6. Application of Hydrogen/Deuterium Exchange Mass Spectrometry in Structural Biology and Molecular Medicine. A chapter in the Book Advances in Biochemistry and Applications in medicineVolume-2; Bindu Yadaganahalli Srinivasu, Amit Kumar Mandal http://openaccessebooks.com/biochemistry-applications-in-medicine-volume-2.html 7. Application of Mass Spectrometry to Analyse Protein Structure within a Live Cell. A chapter in the Book Advances in Biochemistry and Applications in Medicine Vol. 3 http://openaccessebooks.com/biochemistry-applications-in-medicine-volume-3.html. Bindu Yadaganahalli Srinivasu, Amit Kumar Mandal 8. Sickle Cell Hemoglobin. A chapter in the Book Vertebrate and Invertebrate Respiratory Proteins, Lipoproteins and other Body Fluid ProteinsSubcellular Biochemistry, 2020, Volume 94, pages 297-322. Amit Kumar Mandal, Amrita Mitra, Rajdeep Das 9. Mass Spectrometry Imaging: Principles and Protocols. Methods in Molecular Biology, 656. Stainslav S. Rubakhin, Jonathan V. Sweedler |
Course Credit Options
| Sl. No. | Programme | Semester No | Course Choice |
|---|---|---|---|
| 1 | IP | 2 | Elective |
| 2 | IP | 4 | Elective |
| 3 | MP | 2 | Not Allowed |
| 4 | MP | 4 | Not Allowed |
| 5 | MR | 2 | Elective |
| 6 | MR | 4 | Elective |
| 7 | MS | 10 | Elective |
| 8 | MS | 4 | Not Allowed |
| 9 | MS | 6 | Not Allowed |
| 10 | MS | 8 | Elective |
| 11 | RS | 1 | Elective |
| 12 | RS | 2 | Elective |