Details of CH4230 (Spring 2027)
| Level: 4 | Type: Theory | Credits: 4.0 |
| Course Code | Course Name | Instructor(s) |
|---|---|---|
| CH4230 | Instrumentation in Chemical Biology | _ Not Yet Decided _ |
| Preamble |
|---|
| This course is designed to provide students with hands-on proficiency with high-precision tools for observing, measuring, and modeling biomolecules. The course aims to bridge the gap between theoretical physics and biological applications. The student will learn how to extract quantitative data from complex systems |
| Syllabus |
|---|
| Fluorescence Spectroscopy
To study fluorescence emission, excitation spectra, and quenching. Plot Stern-Volmer plot: F0/F vs [Q]. Circular Dichroism (CD) Spectroscopy To estimate protein secondary structure. CD measures differential absorption of left- and right-circularly polarized light. Identify alpha-helix, beta-sheet, random coil signatures. Dynamic Light Scattering (DLS) To determine the hydrodynamic radius of biomolecules. Chromatography Separation: Use of HPLC (e. g. quantifying caffeine in beverages or quantifying prescription drugs in a combination) Characterization of small molecules by mass spectroscopy and NMR spectroscopy Studying Antibody-Antigen Binding Molecular Modeling To visualize protein structures using PyMOL/Chimera and study small molecule-protein interactions (Docking) |
| References |
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| Study materials will be provided in class |
Course Credit Options
| Sl. No. | Programme | Semester No | Course Choice |
|---|---|---|---|
| 1 | IP | 2 | Not Allowed |
| 2 | IP | 4 | Not Allowed |
| 3 | MP | 2 | Not Allowed |
| 4 | MP | 4 | Not Allowed |
| 5 | MR | 2 | Not Allowed |
| 6 | MR | 4 | Not Allowed |
| 7 | MS | 10 | Not Allowed |
| 8 | MS | 4 | Not Allowed |
| 9 | MS | 6 | Not Allowed |
| 10 | MS | 8 | Not Allowed |
| 11 | RS | 1 | Not Allowed |
| 12 | RS | 2 | Not Allowed |