One of the key areas we will be exploring more will be the local crosstalk of different endocytic pathways - clathrin mediated, caveolae etc AND understanding mechanical and molecular remodelling on the membrane by cell stretching. Image analysis (MATLAB and Python-based) with emphasis on customized codes are important for these studies.
Clustering of receptors is key in various signaling pathways as well endocytotis pathways. In many cases molecular ineractions between receptors is not suffiecient for building bigger clusters. Can membrane fluctuations control protein clustering? Suggested for Shiga Toxin by others. We check this across systems from signalling molecules like TNFR1 (collaboration Deepak Sinha, IACS) to receptors involved in intiating endocytosis (Transferrin receptor) as well as CTR1 - the Cu transporter (collaboration Arnab Gupta, DBS).
The other very close to our heart problem is to understand how mechanbical perturbations flow in cells and whether cells have designed to control the flow by making membrane invaginations (caveolae) become tension-sensistive and whether the flow matters - for example does faster flow render mechanoprotection to cells? How does membrane-actin interaction via Ezrin impact it?
While tackling these questions in cells - single cells - we are also intrigued by new rules of motility (collaboration Dipjyoti Das, DBS) and different role of membrane tension in collective cell migration. Are flows also controlled?
Now longstanding colboration with Dipyaman Ganguly helps us explore role of mechanics in immune cells.
PUBLICATIONS:
2024
Review Articles:
The Plasma Membrane and Mechanoregulation in Cells
Upasana Mukhopadhyay, Tithi Mandal, Madhura Chakraborty, and Bidisha Sinha
ACS Omega Article ASAP
DOI: 10.1021/acsomega.4c01962
Research Articles:
Published: Sept 2024
Parijat Biswas, Priyanka Roy, Subhamoy Jana, Dipanjan Ray, Jibitesh Das, Bipasa Chaudhuri, Ridita Ray Basunia, Bidisha Sinha, Deepak Kumar Sinha (2024) Exploring the role of macromolecular crowding and TNFR1 in cell volume control eLife 13:e92719
https://doi.org/10.7554/eLife.92719
Published: Jan 2024
Mandal T, Biswas A, Ghosh T, Manikandan S, Kundu A, Banerjee A, Mitra D, Sinha B. Mechano-regulation by clathrin pit-formation and passive cholesterol-dependent tubules during de-adhesion. Cell Mol Life Sci. 2024 Jan 13;81(1):43. doi: 10.1007/s00018-023-05072-4. PMID: 38217571; PMCID: PMC10787898.
https://link.springer.com/article/10.1007/s00018-023-05072-4
2023
Research Articles:
Published: Oct 2023
Liu Chinky Shiu Chen, Mandal Tithi, Biswas Parijat, Hoque Md. Asmaul, Bandopadhyay Purbita, Sinha Bishnu Prasad, Sarif Jafar, D’Rozario Ranit, Sinha Deepak Kumar, Sinha Bidisha, Ganguly Dipyaman (2023) Piezo1 mechanosensing regulates integrin-dependent chemotactic migration in human T cells eLife 12:RP91903
https://doi.org/10.7554/eLife.91903.1
Published: Oct 2023
Sharma M, Mukherjee S, Shaw AK, Mondal A, Behera A, Das J, Bose A, Sinha B, Sarma JD. Connexin 43 mediated collective cell migration is independent of Golgi orientation. Biol Open. 2023 Oct 15;12(10):bio060006. doi: 10.1242/bio.060006. Epub 2023 Oct 30. PMID: 37815438; PMCID: PMC10629497.
2022
Research Articles:
Published: Oct 2022
Chakraborty M, Sivan A, Biswas A and Sinha B (2022) Early tension regulation coupled to surface myomerger is necessary for the primary fusion of C2C12 myoblasts. Front. Physiol. 13:976715. doi: 10.3389/fphys.2022.976715
Published: 8th March 2022
Effect of heterogeneous substrate adhesivity of follower cells on speed and tension profile of leader cells in primary keratocyte collective cell migration
Madhura Chakraborty, Baishali Mukherjee, Nanditha Nalinakshan, Arikta Biswas, Rajesh Kumble Nayak, Bidisha Sinha
Biol Open (2022) 11 (3): bio058893. https://doi.org/10.1242/bio.058893
Featured on Cover:
Archived:
[Submitted August 2024]
Intra-cluster receptor density (IRD) dictates TNFR1 clusters’ signaling efficacy
Subhamoy Jana, Priyanka Roy, Jibitesh Das, Parijat Biswas, Nandana Nanda, Bidisha Sinha, Deepak Kumar Sinha
bioRxiv 2024.08.09.607302; doi: https://doi.org/10.1101/2024.08.09.607302
[Submitted on 31 May 2023]
Formation of clathrin-pits and ATP-independent cholesterol-dependent tubules initiates mechano-regulation on de-adhesion
Tithi Mandal, Arikta Biswas, Tanmoy Ghosh, Sreekanth Manikandan, Avijit Kundu, Ayan Banerjee, VDhrubaditya Mitra, Bidisha Sinha
doi: https://doi.org/10.1101/2023.05.31.543020
[Submitted on 25 May 2022]
Estimate of entropy generation rate can spatiotemporally resolve the active nature of cell flickering
Sreekanth K Manikandan, Tanmoy Ghosh, Tithi Mandal, Arikta Biswas, Bidisha Sinha, Dhrubaditya Mitra
arXiv:2205.12849 [cond-mat.soft]
https://doi.org/10.48550/arXiv.2205.12849
> now accepted at PRR
Probing the role of macromolecular crowding in cell volume regulation using fluorescence anisotropy
Parijat Biswas, Dipanjan Ray, Subhamoy Jana, Jibitesh Das, Bidisha Sinha, Deepak Kumar Sinha
doi: https://doi.org/10.1101/2022.04.07.487477
2021:
Review Article
Published: 23 January 2021
Membrane Homeostasis: The Role of Actin Cytoskeleton
Arikta Biswas, Rinku Kumar and Bidisha Sinha
Journal of the Indian Institute of Science volume 101, pages81–95(2021)
2019:
Cell spread area and traction forces determine myosin-II-based cortex thickness regulation
R Kumar, S Saha, B Sinha
Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 17 2019
Dystrophy-associated caveolin-3 mutations reveal that caveolae couple IL6/STAT3 signaling with mechanosensing in human muscle cells
M Dewulf, DV Köster, B Sinha, CV de Lesegno, V Chambon, A Bigot, ...
Nature communications 10 (1), 1974 32 2019
Cholesterol depletion by MβCD enhances cell membrane tension, its variations and reduces integrity
A Biswas, P Kashyap, S Datta, T Sengupta, B Sinha
Biophysical Journal
2018:
Cellular and Nuclear Forces: An Overview
B Sinha, A Biswas, GV Soni
Molecular Motors, 1-29
2017:
Mapping cell membrane fluctuations reveals their active regulation and transient heterogeneities
A Biswas, A Alex, B Sinha
Biophysical journal 113 (8), 1768-1781
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