Amit Kumar Mandal

Associate Professor
Dept: Biological Sciences (DBS)
E-mail: amitkm [at] iiserkol.ac.in

All Publications:

  1. 56. Mathew, Boby; Srinivasan, Krishnamachari; Pradeep, Johnson; Thomas, Tinku and Mandal, Amit Kumar. 2021."Plasma Apolipoprotein-AIV is down regulated in patients with major depressive disorder having suicidal ideation compared to those without suicidal ideation ." Current Proteomics , In press, In press
  2. 55. Srinivasu, Bindu Y; Arumugaperumal, Arun; Mitra, Amrita; Muralidharan, Monita; Das, Rajdeep and Mandal, Amit Kumar. 2021."Effect of nanoparticle exposure in a living system using: probed by quantification of Fetuin-B in plasma proteome and imaging of kidney tissue using MALDI Imaging Mass Spectrometry in a rat model." Journal of Nanoparticle Research, 23, Article125
  3. 54. Choubey, Surya Kant; Mitra, Amrita; Das, Rajdeep; Rout, Pritilata and Mandal, Amit Kumar. 2021."Molecular portrait of prostate tissue probed by MALDI Imaging Mass Spectrometry." medRxiv, pre print , pre print
  4. 53. Mitra, Amrita; Das, Rajdeep; Choubey, Surya Kant and Mandal, Amit Kumar. 2021."Molecular analysis of prostate cancer: prostate tissue and urine proteomics based approach." bioRxiv, pre print, pre print
  5. 52. Debnath, Utsab; Mitra, Amrtia; Dewakar, Varun; Prabhakar, Yenamandra S; Tadala, Raghu; Krishnan, Kiran; Wagh, Padmakar; Velusamy, Umashankar; Subramani, Chandru; Agarwal, Shubhra; Vrati, Sudhanshu; Baliyan, Aastha; Kurpad, Anura V; Bhattacharyya, Parthasarathi and Mandal, Amit Kumar. 2021."N-acetyl cysteine: A tool to perturb SARS-CoV-2 spike protein conformation." ChemRxiv, preprint, preprint
  6. 51. Mitra, Amrita; Choubey, Surya Kant; Raut, Pritilata; Sridhar, T S; Mishra, Dipak and Mandal, Amit Kumar. 2020."Differential expression of proteins in human prostate cancer tissues probed by MALDI imaging mass spectrometry." bioRxiv, preprint, preprint
  7. 50. Narayanan, Sreekala; Mathew, Boby; Srinivasu, Bindu Y; Bhat, Vijay; Ross, Cecil and Mandal, Amit Kumar. 2020."Effect of Point Mutation on Structure-Function Correlation of Hemoglobin Variants, HbE and HbD Punjab." Amino acids, 52, 893-904
  8. 49. Devi, Sarita; Pasannaa, Roshni M; Shamshuddin, Zeeshan; Bhat, Kishor; Ambily, Sivadas; Mandal, Amit Kumar and Kurpad, Anura V. 2020."Measuring vitamin B12 bioavailability with[13C]-cyanocobalamin in humans." American Journal of Clinical Nutrition, in press, in press
  9. 48. Das, Rajdeep; D’souza, Nisha; Choubey, Surya Kant; Murlidharan, Sethumadhavan; Kurpad, Anura V and Mandal, Amit Kumar. 2020."Analysis of extracellular proteome of Staphylococcus aureus: A mass spectrometry based proteomics method of exotoxin characterization." Current Proteomics, 17, 3-9
  10. 47. Narayanan, Sreekala; Mathew, Boby; Srinivasu, Bindu Y; Muralidharan, Monita; Bhat, Vijay and Mandal, Amit Kumar. 2020."Functional Prediction from Conformational Dynamics of Glycated and Glutathionylated HbE and HbD Punjab." Current Science, 118, 722
  11. 46. Muralidharan, Monita; Bhat, Vijay and Mandal, Amit Kumar. 2019."Structural analysis of glycated hemoglobin using native mass spectrometry." FEBS J, 287, 1247-1254
  12. 45. Mathew, Boby; Srinivasan, Krishnamachari; Johnson, Pradeep; Thomas, Tinku; Murthy, Shakunthala Kandikuppa and Mandal, Amit Kumar. 2019."Down regulation of Apolipoprotein-AIV in plasma and impaired reverse cholesterol transport in individuals with recent acts of deliberate self-harm." Indian Journal of Medical Research, 150, 365
  13. 44. Muralidharan, Monita; Mitra, Amrita; Maity, Dibyajyoti; Pal, Debnath and Mandal, Amit Kumar. 2019."Structural analysis of glutathionylated hemoglobin using native mass spectrometry." Journal of Structural Biology , 208, 107386
  14. 43. Mathew, Boby; Srinivasan, Krishnamachari; Johnson, Pradeep; Thomas, Tinku and Mandal, Amit Kumar. 2019."Elevated Levels of Glutathionyl Hemoglobin as an Oxidative Stress Marker in Patients with Major Depressive Disorder ." Indian Journal of Medical Research, 149, 497
  15. 42. Muralidharan, Monita; Bhat, Vijay; Srinivasu, Bindu Y and Mandal, Amit Kumar. 2019."Glycation profile of minor abundant erythrocyte proteome across varying glycemic index in Diabetes Mellitus." Analytical Biochemistry, 573, 37
  16. 41. Jebarupa, Benita; Mathew, Boby; Srinivasu, Bindu Y; Sasikumaran, Aiswarya; Mandal, Amit Kumar and Mitra, Gopa. 2019."Understanding molecular basis of aggregation-resistant of tau conformation using oxidized monomer." BBA - General Subjects, 1863, 993
  17. 40. Srivastava, Deepsikha; Sarma, Gosala Raja Kukkuta; D’Souza, Dilon; Muralidharan, Monita; Srinivasan, Krishnamachari and Mandal, Amit Kumar. 2019."Characterization of residue-specific glutathionylation of CSF proteins in multiple sclerosis – A MS-based approach." Analytical Biochemistry, 564-565, 108
  18. 39. Das, Rajdeep and Mandal, Amit Kumar. 2018."Genetic variants of hemoglobin in hemoglobinopathies with functional disorder." International Journal of Clinical and Medical Informatics, 1, 59
  19. 38. Jebarupa, Benita; Muralidharan, Monita; Arun, Arumugaperumal; Mandal, Amit Kumar and Mitra, Gopa. 2018."Conformational heterogeneity of tau: Implication on inherent disorder and fibrillation in Alzheimer’s disease." Biophysical Chemistry, 241, 27
  20. 37. Das, Rajdeep; Mitra, Amrita; Mitra, Gopa; Maity, Dibyajyoti; Bhat, Vijay; Pal, Debnath; Ross, Cecil; Kurpad, Anura V and Mandal, Amit Kumar. 2018."Molecular insights of inhibition in sickle hemoglobin polymerization upon glutathionylation: hydrogen/deuterium exchange mass spectrometry and molecular dynamics simulation-based approach." Biochemical Journal, 475, 2153
  21. 36. Mitra, Amrita and Mandal, Amit Kumar. 2018."Conjugation of para-benzoquinone of Cigarette Smoke with Human Hemoglobin Leads to Unstable Tetramer and Reduced Cooperative Oxygen Binding." Journal of the American Society for Mass Spectrometry, 29, 2048
  22. 35. Pauline, Maria; Verghese, Sejil T; Srinivasu, Bindu Y; Bose, Beena; Thomas, Tinku; Mandal, Amit Kumar; Thankachan, Prashanth and Kurpad, Anura V. 2018."Effect of ascorbic acid rich, micro-nutrient fortified supplement on the iron bioavailability of ferric pyrophosphate from a milk based beverage in Indian school children." Asia Pacific Journal of Clinical Nutrition, 27, 792
  23. 34. Pyne, Prosenjit; Alam, Masrure; Rameez, Moidu Jameela; Mandal, Subhrangshu; Mondal, Nibendu; Sar, Abhijit; Debnath, Utsab; Mathew, Boby; Misra, Anup Kumar; Mandal, Amit Kumar and Ghosh, Wriddhiman. 2018."Homologs from sulfur oxidation (Sox) and methanol dehydrogenation (Xox) enzyme systems collaborate to give rise to a novel pathway of chemolithotrophic tetrathionate oxidation." Molecular Microbiology, 109, 169
  24. 33. Jebarupa, Benita; Muralidharan, Monita; Srinivasu, Bindu Y; Mandal, Amit Kumar and Mitra, Gopa. 2018."Effect of altered solution conditions on tau conformational dynamics: Plausible implication on order propensity and aggregation." BBA - Proteins and Proteomics, 1866, 668
  25. 32. Muralidhran, Monita; Das, Rajdeep; Bhat, Vijay and Mandal, Amit Kumar. 2018."Analysis of the Quaternary Structure of Hemoglobin Beckman Variant and Molecular Interpretation of Its Functional Abnormality: A Mass-Spectrometry-Based Approach." ChemBioChem, 19, 1
  26. 31. Mathew, Boby; Srinivasan, Krishnamachari; Johnson, Pradeep; Thomas, Tinku and Mandal, Amit Kumar. 2018."Suicidal behaviour is associated with decreased esterified cholesterol in plasma and membrane fluidity of platelets." Asian Journal of Psychiatry, 32, 105
  27. 30. Mitra, Amrita; Muralidharan, Monita; Srivastava, Deepsikha; Das, Rajdeep; Bhat, Vijay and Mandal, Amit Kumar. 2017."Assessment of cysteine reactivity of human hemoglobin at its residue level: A mass spectrometry based approach." Hemoglobin, 41, 300
  28. 29. Arun, Arumugaperumal; Sreekala, Sreekala; Sarangi, Subhasis and Mandal, Amit Kumar. 2017."Analysis of isotopic peaks in hydrogen/deuterium exchange mass spectra for precise measurement of isotope average centroid mass of peptides and proteins." International Journal of Biochemistry Research & Review, 18, 1
  29. 28. Srinivasu, Bindu Y; Bose, Beena; Mitra, Gopa; Kurpad, Anura V and Mandal, Amit Kumar. 2017."Molecular insights of inhibition in sickle hemoglobin polymerization upon glutathionylation: hydrogen/deuterium exchange mass spectrometry and molecular dynamics simulation-based approach." Langmuir, 33, 8032
  30. 27. Das, Rajdeep; Mitra, Amrita; Bhat, Vijay and Mandal, Amit Kumar. 2017."Application of isotope exchange based mass spectrometry to understand the mechanism of inhibition of sickle hemoglobin polymerization upon oxygenation." Journal of Structural Biology, 199, 76
  31. 26. Das, Rajdeep; Mitra, Gopa; Mathew, Boby; Bhat, Vijay; Ross, Cectl; Pal, Debnath and Mandal, Amit Kumar. 2016."Mass spectrometry-Based Diagnosis of Hemoglobinopathies: A Potential Tool for the Screening of Genetic Disorder." Biochemical Genetics, 54, 816
  32. 25. Ghosh, Arunava; Banerjee, Santanu; Mitra, Amrita; Muralidharan, Monita; Roy, Bappaditya; Banerjee, Rajat; Mandal, Amit Kumar and Chatterjee, Indu Bhusan. 2016."Interaction of p-benzoquinone with hemoglobin in smoker’s blood causes alteration of structure and loss of oxygen binding capacity." Toxicology Reports , 3, 295
  33. 24. Narayanan, Sreekala; Mitra, Gopa; Muralidharan, Monita; Mathew, Boby and Mandal, Amit Kumar. 2015."Protein Structure Function Correlation in Living Human Red Blood Cells Probed by Isotope Exchange-based Mass Spectrometry." Analytical Chemistry, 87, 11812
  34. 23. Das, Rajdeep; Muralidharan, Monita; Mitra, Gopa; Bhat, Vijay; Mathew, Boby; Pal, Debnath; Ross, Cecil and Mandal, Amit Kumar. 2015."Mass spectrometry based characterization of Hb Beckman variant in a falsely elevated HbA1C sample." Analytical Biochemistry, 489, 53
  35. 22. Srinivasu, Bindu Y; Mitra, Gopa; Muralidharan, Monita; Srivastava, Deepsikha; Pinto, Jennifer; Thankachan, Prashanth; Suresh, Sudha; Shet, Arun; Rao, Suguna; Ravikumar, Gayatri; Thomas, Tinku S; Kurpad, Anura V and Mandal, Amit Kumar. 2015."Beneficiary effect of nanosizing ferric pyrophosphate as food fortificant in iron deficiency anemia: evaluation of bioavailability, toxicity and plasma biomarker." Royal Society of Chemistry Advances, 5, 61678
  36. 21. Bhat, Vijay S; Lokeshwarappa, S and Mandal, Amit Kumar. 2015."Characterization of a Double Heterozygote HbE/?+ Thalassemia IVS 1-1 [G>T] in a Juvenile Diabetic." Indian Journal of Clinical Biochemistry, 30, 238
  37. 20. Vanjari, Siva Rama Krishna; Bhat, Navakanta; Srinivasan, Sampath; Amrutur, Bharadwaj; Kalapu, Chakrapani and Mandal, Amit Kumar. 2014."Title of the Patent: Low cost electrochemical disposable sensor for measuring glycated hemoglobin." United States Patent, No.: US 8,702,931 B2, pp: 1-12
  38. 19. Das, Rajdeep; Mitra, Gopa; Mathew, Boby; Ross, Cecil; Bhat, Vijay and Mandal, Amit Kumar. 2013."Automated Analysis of Hemoglobin Variants Using NanoLC-MS and Customized Databases." Journal of Proteome Research, 12, 3215
  39. 18. Mitra, Gopa; Muralidharan, Monita; Narayanan, Sreekala; Pinto, Jeniffer; Srinivasan, Krishnamachari and Mandal, Amit Kumar. 2012."Glutathionylation induced Structural Changes in Oxy Human Hemoglobin Analyzed by Backbone Amide Hydrogen/Deuterium Exchange and MALDI-Mass Spectrometry." Bioconjugate Chemistry, 23, 2344
  40. 17. Bhat, Vijay; Mathew, Boby and Mandal, Amit Kumar. 2012."Identification of a Rare Hemoglobin Variant HbJ-Rajappen [alpha90 (FG2)Lys ? Thr] Using Mass Spectrometry ." Indian Journal of Clinical Biochemistry, 27, 414
  41. 16. Bhat, Vijay; Mandal, Amit Kumar and Mathew, Boby. 2012."Co-inheritance of HbD Iran /Beta Thalassemia IVS1-5 (G>C) Trait in a Punjabi Lady with Diabetes." Indian Journal of Clinical Biochemistry, 27, 202
  42. 15. Shet, Arun; Pinto, Sneha M; Mitra, Gopa and Mandal, Amit Kumar. 2012."Glutathionyl Hemoglobin is Elevated in Iron Deficiency Anemia ." Acta Haematologica, 127, 26
  43. 14. Mathew, Boby; Bhat, Vijay and Mandal, Amit Kumar. 2011."Analysis of hemoglobin variants using nondenaturing gel electrophoresis and matrix-assisted laser desorption ionization mass spectrometry ." Analytical Biochemistry, 416, 135
  44. 13. Mitra, Gopa; Muralidharan, Monita; Pinto, Jennifer; Srinivasan, Krishnamachari and Mandal, Amit Kumar. 2011."Structural Perturbation of Human Hemoglobin on Glutathionylation Probed by Hydrogen-Deuterium Exchange and MALDI Mass Spectrometry." Bioconjugate Chemistry, 22, 785
  45. 12. Bhat, Vijay S; Dewan, K K; Krishnaswamy, Patnam Rajagopalan; Mandal, Amit Kumar and Balaram, Padmanabhan. 2010."Characterization of a Hemoglobin Variant: HbQ-India/IVS 1-1 [G >T] - ?-Thalassemia." Indian Journal of Clinical Biochemistry, 25, 99
  46. 11. Woodi, Murli; Mandal, Amit Kumar; Balaram, Padmanabhan and Krishnaswamy, Patnam Rajagopalan. 2009."Analysis of Protein Post-translational Modifications by Mass Spectrometry: with special reference to Hemoglobin." Indian Journal of Clinical Biochemistry, 24, 23
  47. 10. Mandal, Amit Kumar; Bisht, Shveta; Bhat, Vijay; Krishnaswamy, Patnam Rajagopalan and Balaram, Padmanabhan. 2008."Electrospray mass spectrometric characterization of hemoglobin Q (Hb Q-India) and a double mutant hemoglobin S/D in clinical samples." Clinical Biochemistry, 41, 75
  48. 9. Mandal, Amit Kumar and Balaram, Padmanabhan. 2007."Mass spectrometric identification of pyroglutamic acid in peptides following selective hydrolysis ." Analytical Biochemistry, 310, 118
  49. 8. Mandal, Amit Kumar; Woodi, Murali; Sood, Varun; Krishnaswamy, Patanam Rajagopalan; Rao, Anjali; Ballal, Sudarshan and Balaram, Padmanabhan. 2007."Quantitation and characterization of glutathionyl hemoglobin as an oxidative stress marker in chronic renal failure by mass spectrometry." Clinical Biochemistry, 40, 986
  50. 7. Mandal, Amit Kumar; Ramasamy, Mani Ramakrishnan Santhana; Sabareesh, Varatharajan; Openshaw, Mathew E; Krishnan, Kozhalmannom S and Balaram, Padmanabhan. 2007."Sequencing of T-Superfamily Conotoxins from Conus Virgo: Pyroglutamic acid Identification and Disulfide Arrangement by MALDI Mass Spectrometry ." Journal of American Society for Mass Spectrometry, 18, 1396
  51. 6. Samaddar, Soma; Mandal, Amit Kumar; Mondal, Sudip Kumar; Sahu, Kalyanasis; Bhattacharyya, Kankan and Roy, Siddhartha. 2006."Solvation Dynamics of a Protein in the Pre Molten Globule State ." Journal of Physical Chemistry B, 110, 21210
  52. 5. Banerjee, Rajat; Mandal, Amit Kumar; Saha, Rajesh; Guha, Soumi; Samaddar, Soma; Bhattacharyya, Anusree and Roy, Siddhartha. 2003."Solvation change and ion release during aminoacylation by aminoacyl-tRNA synthetases ." Nucleic Acids Research, 31, 6035
  53. 4. Mandal, Amit Kumar; Samaddar, Soma; Banerjee, Rajat; Lahiri, Simanti; Bhattacharyya, Anusree and Roy, Siddhartha. 2003."Glutamate Counteracts the Denaturing Effect of Urea through Its Effect on the Denatured State." Journal of Biological Chemistry, 278, 36077
  54. 3. Mandal, Debabrata; Sen, Sobhan; Sukul, Dipankar; Bhattacharyya, Kankan; Mandal, Amit Kumar; Banerjee, Rajat and Roy, Siddhartha. 2002."Solvation Dynamics of a Probe Covalently Bound to a Protein and in an AOT Microemulsion: 4-(N-Bromoacetylamino)-Phthalimide." Journal of Physical Chemistry B, 106, 10741
  55. 2. Bhattacharyya, Anusree; Mandal, Amit Kumar; Banerjee, Rajat and Roy, Siddhartha. 2000."Dynamics of compact denatured states of glutaminyl-tRNA synthetase probed by bis-ANS binding kinetics." Biophysical Chemistry, 87, 201
  56. 1. Mandal, Amit Kumar; Bhattacharyya, Anusree; Bhattacharyya, Subhra; Bhattacharyya, Tista and Roy, Siddhartha. 1998."A cognate tRNA specific conformational change in glutaminyl-tRNA synthetase and its implication for specificity." Protein Science, 7, 1046


  1. 3. Mandal, Amit Kumar; Mitra, Amrita and Das, Rajdeep. 2020." Sickle Cell Hemoglobin", "Subcellular Biochemistry: Vertebrate and Invertebrate Respiratory Proteins, Lipoproteins and other Body Fluid Proteins". © Springer Nature Switzerland AG 2020, ISBN: 9783030417697
  2. 2. Srinivasu, Bindu Y and Mandal, Amit Kumar. 2018." Application of Mass Spectrometry to Analyse Protein Structure within a Live Cell", "Advances in Biochemistry and Applications in Medicine (Volume 3)". Open Access eBooks; 848 N. Rainbow Blvd, #5486, Las Vegas, NV 89107, USA, E-mail: info@openaccessebooks.com, ISBN: 9789387500228
  3. 1. Srinivasu, Bindu Y and Mandal, Amit Kumar. 2017." Application of Hydrogen/Deuterium Exchange Mass Spectrometry in Structural Biology and Molecular Medicine", "Advances in Biochemistry and Applications in Medicine (Volume 2)". Open Access eBooks; 848 N. Rainbow Blvd, #5486, Las Vegas, NV 89107, USA, E-mail: info@openaccessebooks.com, ISBN: 9789387500198