Rupak Datta

Associate Professor
Dept: Biological Sciences (DBS)
E-mail: rupakdatta [at] iiserkol.ac.in
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Research Interest:
 

Lysosomes are cell's recycling and waste disposal system. It contains a battery of enzymes (acid hydrolases) that degrade a wide variety of macromolecules and cellular debris into reusable forms. In so doing lysosomes play a vital role in maintaining cellular homeostasis. The broad goal of our research is to understand the role of this fascinating organelle in human health and diseases. We use various methods ranging from molecular biology, cell biology, protein biochemistry to genomic and proteomic tools to address our research problems. Current research in our laboratory is focused on two major areas:1. To investigate the pathogenesis of mucopolysaccharidoses, a group of genetic disorders caused by deficiency of one of the eleven lysosomal enzymes required for stepwise degradation of glycosaminoglycans (GAGs). Widespread lysosomal accumulation of undegraded or partially degraded GAGs results in cellular and multiple organ dysfunctions leading to premature death in most cases. The genetic causes of these diseases despite being known for years, actual pathways leading from lysosomal storage to cellular damage or death are still unclear. We address these unresolved issues by using well-characterized cell culture and mouse models of these diseases. We are also trying to develop a drosophila model of these diseases.2. To understanding how certain intracellular pathogens utilize lysosome as their replicating niche. Phagocytes normally use acidic and proteolytic environment of the lysosome to destroy engulfed pathogens. But, some pathogens are smart enough to be able to survive and even replicate in such harsh conditions. Using a macrophage infection model of the protozoan parasite Leishmania sp. we ask: a) how the pathogens tolerate such harsh lysosomal conditions and b) how replicating parasites compete with the host for acquisition of essential nutrients (e.g. iron).

Academic Background:

  1. PhD (Biochemistry & Molecular Biology), Indian Institute of Chemical Biology, 2006
  2. M.Sc (Biochemistry), University of Calcutta, 2000

Positions:

  1. Associate Professor, IISER Kolkata ( - )
  2. Assistant Professor, IISER Kolkata (2010 - 2017)
  3. DBT-Ramalingaswami Fellow, IISER Kolkata (2011 - 2016)
  4. Postdoctoral Fellow, Saint Louis University (2006 - 2010)

Awards and Honors:

  1. Young Investigator Award from International Congress of Human Genetics, Montreal (2011)
  2. Ramalingaswami Fellowship from Department of Biotechnology, Government of India (2011)
  3. Travel award from Protein Mis-folding and Mis-processing in Disease meeting, National Institutes of Health (2009)
  4. Postdoctoral Presentation Finalist Award from American Society of Human Genetics, San Diego (2007)

Selected Publications:

  1. Bar, Sudipta; Prasad, Mohit and Datta, Rupak. 2018."Neuromuscular degeneration and locomotor deficit in a Drosophila model of mucopolysaccharidosis VII is attenuated by treatment with resveratrol." Disease Models & Mechanisms , 11, 1 - 4
  2. Pal, Dhiman Sankar; Abbasi, Mazharul; Mondal, Dipon Kumar; Varghese, Binitha Anu; Paul, Ritama; Singh, Shalini and Datta, Rupak. 2017."Interplay between a cytosolic and a cell surface carbonic anhydrase in pH homeostasis and acid tolerance of Leishmania." Journal of Cell Science, 130, 754-766
  3. Pal, Dhiman Sankar; Mondal, Dipon Kumar and Datta, Rupak. 2015."Identification of metal dithiocarbamates as a novel class of antileishmanial agents." Antimicrobial Agents and Chemotherapy, 59, 2144 - 52
  4. Datta, Rupak; Shah, Gul N; Rubbelke, Timothy S; Waheed, Abdul; Rauchman, Michael; Goodman, Alan G; Katze, Michael G and Sly, William S. 2010."Progressive renal injury from transgenic expression of human carbonic anhydrase IV folding mutants is enhanced by deficiency of p58IPK." PNAS, 107, 6448-52

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